核糖體序列標簽連續分析(serial analysis of ribosomal sequence tag, SARST),是一種高通量分子生態學技術,將環境中的rRNA中某段高變異性的序列(如V6高變區)連接到一起,一併測序的方法。其優點爲,可以高效率地測定環境中的物種多樣性,更易檢測到出現頻率低的rRNA。
参考文献
外部連結
*[http://aem.asm.org/cgi/content/abstract/68/12/6077 Sequencing independent method to generate oligonucleotide probes targeting a variable region in bacterial 16S rRNA by PCR with detachable primers]
*Serial analysis of ribosomal sequence tags (SARST): a new high-throughput method for profiling complex microbial communities
*Serial analysis of V6 ribosomal sequence tags (SARST-V6): a new method for efficient, high-throughput analysis of microbial community composition
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